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    Downstream Processing: Ultrafiltration and Diafiltration

    Describes the downstream manufacturing processes of ultrafiltration and diafiltration with an emphasis on post-harvest volume reduction and concentration for therapeutic protein products. The components of an UF/DF skid and control of the UF/DF process are also described.

      Duration: 30 Mins

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    SKU: BPU-1102-SPL03-EN Category: Tag:

    Description

    Learning Objectives:

    • Explain the role of filtration in protein purification methods
    • Describe the role of ultrafiltration / diafiltration (UF/DF) in downstream processing
    • Describe the difference between Normal Flow Filtration (NFF) and Tangential Flow Filtration (TFF)
    • List the advantages of TFF over NFF
    • Explain the terms molecular weight cut off and transmembrane pressure
    • Identify important components of a UF/DF skid and their functions
    • List and explain the critical process parameters involved in a UF/DF operation
    • Describe the in-process checks and quality tests that are carried out during a UF/DF operation
    • Describe how a UF/DF skid is cleaned
    • Describe the safety requirements associated with UF/DF
    • List some common problems encountered when operating UF/DF
    		 Module Features:

        Animations
       Voice Over
        Knowledge Checks
        Assessments
       SCORM/AICC compatible
        Full Screen

     

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