BPU 802 01

Downstream Processing: Ultrafiltration and Diafiltration


Per License / Per Trainee  –  (1 Lesson)

This module describes the downstream manufacturing processes of ultrafiltration and diafiltration with an emphasis on post-harvest volume reduction and concentration for therapeutic protein products.


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– Describe how UF/DF is used in downstream processing
– Describe the different uses for UF/DF
– Describe the in-process checks and quality tests that are carried out during an UF/DF operation
– Describe the safety and requirements associated with UF/DF equipment
– Describe what is meant by ultrafiltration and diafiltration (UF/DF)
– List and explain the critical process parameters involved in an UF/DF operation
– List and explain the different steps of an UF/DF operation
– Name the key instruments of an UF/DF skid and describe their purpose
– Name the main components of an UF/DF skid and describe their purpose


Duration: 30 mins (lesson content and associated assessment).

Prerequisites: None

Assessment: 10 Multiple Choice Questions.