Cell Biology and Recombinant DNA Technology

Lesson Code: BPU-1108 (PREMIUM LESSON) Following on from BPU-1100, this Lesson goes a level deeper in its explanation of cell biology and how cells can be manipulated to produce therapeutic proteins. An overview is provided of the functioning of mammalian cells, followed by an explanation of the roles played by DNA and RNA in producing proteins in cells. The steps involved in recombinant DNA technology are outlined, including DNA amplification, insertion of target genes into suitable vectors, before cell culturing is explained.

  • Category: Biopharma Manufacturing - Fundamentals

Description

After completing this e-Lesson, you will be able to:

  • Describe the key characteristics of mammalian cells
  • Explain the difference between DNA and RNA
  • Describe the structure of a protein
  • Describe the role of proteins in the human body
  • Explain the terms genetic engineering, recombinant technology and DNA manipulation
  • Describe what is meant by 'vector' and 'plasmid'
  • Differentiate between transfection and infection
  • Describe some of the products that can be produced using recombinant DNA technology