Downstream Processing: Protein Purification - Chromatography

Lesson Code: BPU-810

  • Category: Biopharma Processes and Equipment


Describes the use of various chromatographic methods in downstream protein purification including size exclusion, ion exchange, hydrophobic interaction and affinity chromatographies. The basics of a chromatography set-up are covered along with critical factors affecting protein separation such as column packing, resolution, column capacity, pressure and the gel matrix.