Downstream Processing: Ultrafiltration and Diafiltration

Lesson Code: BPU-802. Describes the downstream manufacturing processes of ultrafiltration and diafiltration with an emphasis on post-harvest volume reduction and concentration for therapeutic protein products. The components of an UF/DF skid and control of the UF/DF process are also described.

  • Category: Biopharma Processes and Equipment


After taking this e-Lesson, you will be able to:

- Describe how Ultrafiltration/Diafiltration is used in downstream processing

- Describe the different uses for Ultrafiltration/Diafiltration

- Describe the in-process checks and quality tests that are carried out during an Ultrafiltration/Diafiltration operation

- Describe the safety requirements associated with Ultrafiltration/Diafiltration equipment

- Describe what is meant by Ultrafiltration and Diafiltration

- List and explain the critical process parameters involved in an Ultrafiltration/Diafiltration operation

- List and explain the different steps of an Ultrafiltration/Diafiltration operation

- Name the key instruments of an Ultrafiltration/Diafiltration skid and their purpose

- Name the main components of an Ultrafiltration/Diafiltration skid and their purpose

Check out these screenshots from the e-Lesson:

You can download the PDF product Brochure here:


EU_Bioprocessing Product Sheet.pdf


US_Bioprocessing Product Sheet.pdf