Lesson Code: BPU-1102 (PREMIUM LESSON) Describes the downstream manufacturing processes of ultrafiltration and diafiltration with an emphasis on post-harvest volume reduction and concentration for therapeutic protein products. The components of an UF/DF skid and control of the UF/DF process are also described.

  • Category: Biopharma Processes and Equipment


After completing this e-Lesson you will be able to:

  • Explain the role of filtration in protein purification methods
  • Describe the role of ultrafiltration / diafiltration (UF/DF) in downstream processing
  • Describe the difference between Normal Flow Filtration (NFF) and Tangential Flow Filtration (TFF)
  • List the advantages of TFF over NFF
  • Explain the terms molecular weight cut off and transmembrane pressure
  • Identify important components of a UF/DF skid and their functions
  • List and explain the critical process parameters involved in a UF/DF operation
  • Describe the in-process checks and quality tests that are carried out during a UF/DF operation
  • Describe how a UF/DF skid is cleaned
  • Describe the safety requirements associated with UF/DF
  • List some common problems encountered when operating UF/DF