After completing this e-Lesson you will be able to:
- Explain the role of filtration in protein purification methods
- Describe the role of ultrafiltration / diafiltration (UF/DF) in downstream processing
- Describe the difference between Normal Flow Filtration (NFF) and Tangential Flow Filtration (TFF)
- List the advantages of TFF over NFF
- Explain the terms molecular weight cut off and transmembrane pressure
- Identify important components of a UF/DF skid and their functions
- List and explain the critical process parameters involved in a UF/DF operation
- Describe the in-process checks and quality tests that are carried out during a UF/DF operation
- Describe how a UF/DF skid is cleaned
- Describe the safety requirements associated with UF/DF
- List some common problems encountered when operating UF/DF
