Lesson Code: PPM-1210. This e-Lesson is aimed at personnel who require an overview of techniques frequently used in microbiology laboratories including media preparation, handling stock cultures, and pure culture techniques.

  • Category: Microbiology Laboratory - Practices


After taking this e-Lesson, you will be able to:

  • Explain the term 'microbiological media' and name the three different media forms
  • Explain the difference between agar plates, agar slants and agar deeps
  • Explain the difference between reserve and working stock cultures
  • Describe how reserve and working stock cultures are used and stored
  • Describe the quadrant and continuous streak plate methods
  • Describe the common streak plate method mistakes
  • Describe the pour plate method

KEYWORDS: Agar Deeps, Agar Plates, Agar Slants, Aseptic Technique, Autoclaving, Continuous Streak, Cryo-Beads, Media, Pour Plate, Quadrant Streak, Stock Culture, Streak Plate