Lesson Code: PPL-1130. (PREMIUM LESSON) Describes the basic theory and practice of HPLC. Particular focus is given to reverse phase HPLC. The components of a HPLC are described in detail and significant consideration is given to the explanation of results and peak shape. Analytical considerations of special relevance to HPLC are also presented.

  • Category: Analytical Laboratory - Practices


After taking this e-Lesson, you will be able to:

- Describe the components of a HPLC column

- Explain the basic concepts behind chromatography

- Describe the meaning of 'partitioning'

- Differentiate between a mobile phase and a stationary phase

- Explain the components of a system diagram of a HPLC

- Explain how phase polarity can assist separation

- List the methods commonly employed for optimization of separation