Lesson Code: PPM-1212. This Lesson is aimed at personnel who require an overview of bacterial staining, including how to prepare a good bacterial smear on a glass slide, how to fix bacterial smears, and the different categories of bacterial stains.

  • Category: Microbiology Laboratory - Practices


After taking this e-Lesson, you will be able to:

  • Explain why cells are stained with dyes and how this aids their study
  • Explain the procedures for preparing smears from liquid and solid cultures
  • List and describe examples of smearing errors
  • Explain the procedure for heat fixing bacterial smears
  • List the different categories of bacterial stains
  • Describe the characteristics of each category of bacterial stain

KEYWORDS: Bacterial Smear, Differential Staining, Heat Fixing, Simple Staining, Structural Staining