Lesson Code: PPM-1200. The importance of good aseptic technique and the major steps involved in applying it to microbiological testing.

  • Category: Microbiology Laboratory - Practices


After taking this e-Lesson, you will be able to:

- Explain why good aseptic technique is necessary in the microbiological laboratory

- Explain the terms 'false positive' and 'positive control'

- Describe the general steps for reducing the risk of contamination in microbiological testing

- List the three common steps that apply to all microbiological handling techniques

- Describe how to flame an inoculating loop

- Describe the correct aseptic technique for handling liquid broths

- Describe the correct aseptic technique for handling agar plates

Keywords: Agar Plate, Aseptic Technique, Culture, False Positive, Liquid Broth, Loop Sterilization, Microorganisms, Positive Control, Tube Sterilization