Staining Techniques

Lesson Code: PPM-1213. This e-Lesson is aimed at personnel who require an overview of the techniques used to stain and identify organisms. The types of staining dyes used are detailed and a number of staining techniques are explored, namely: Simple Staining, Negative Staining, Gram Staining, Acid-Fast Staining, and Spore Staining.

  • Category: Microbiology Laboratory - Practices

Description

After completing this e-Lesson, you will be able to:

  • Explain the terms: 'blotting', 'cover', 'decolorize' and 'stain'
  • Describe the purpose and procedure of simple and negative staining and list examples of the dyes used
  • Describe the purpose and procedure of gram staining and list examples of the dyes used
  • List common gram staining problems
  • Describe the purpose and procedure of acid-fast and spore staining and list examples of the dyes used


KEYWORDS: Acid-Fast Staining, Differential Staining, Gram Staining, Heat Fixing, Negative Staining, Simple Staining, Spore Staining, Structural Staining