Skip to content

Founded in 1998 in Dublin, Ireland, Skillpad's team of industry-experienced Subject Matter Experts and Consultants believes Pharma, Biopharma, and Life Science Industry manufacturing companies can achieve production excellence with improved performance, productivity & compliance, as well as reduced losses & down-time, if they have KNOWLEDGE CONSISTENCY in all critical areas of manufacturing operations.

With 25 years in business, and offices in Europe, the USA and Canada, Skillpad has helped customers all over the world with Life Science Industry Knowledge challenges. We are fully dedicated to the idea that Knowledge Consistency is more achievable than ever using the latest technology and advanced knowledge transfer and management tools.


Get In Touch

    Downstream Processing: Ultrafiltration and Diafiltration

    Describes the downstream manufacturing processes of ultrafiltration and diafiltration with an emphasis on post-harvest volume reduction and concentration for therapeutic protein products. The components of an UF/DF skid and control of the UF/DF process are also described.

      Duration: 30 Mins

    SKU: BPU-1102-SPL03-EN Category: Tag:


    Learning Objectives:

    • Explain the role of filtration in protein purification methods
    • Describe the role of ultrafiltration / diafiltration (UF/DF) in downstream processing
    • Describe the difference between Normal Flow Filtration (NFF) and Tangential Flow Filtration (TFF)
    • List the advantages of TFF over NFF
    • Explain the terms molecular weight cut off and transmembrane pressure
    • Identify important components of a UF/DF skid and their functions
    • List and explain the critical process parameters involved in a UF/DF operation
    • Describe the in-process checks and quality tests that are carried out during a UF/DF operation
    • Describe how a UF/DF skid is cleaned
    • Describe the safety requirements associated with UF/DF
    • List some common problems encountered when operating UF/DF
    Module Features:

       Voice Over
        Knowledge Checks
       SCORM/AICC compatible
        Full Screen


    Back To Top