Skillpad_Logo

Founded in 1998 in Dublin, Ireland, Skillpad's team of industry-experienced Subject Matter Experts and Consultants believes Pharma, Biopharma, and Life Science Industry manufacturing companies can achieve production excellence with improved performance, productivity & compliance, as well as reduced losses & down-time, if they have KNOWLEDGE CONSISTENCY in all critical areas of manufacturing operations.

With 25 years in business, and offices in Europe, the USA and Canada, Skillpad has helped customers all over the world with Life Science Industry Knowledge challenges. We are fully dedicated to the idea that Knowledge Consistency is more achievable than ever using the latest technology and advanced knowledge transfer and management tools.

Ireland
USA
Canada
+353-1-687-0400
+1-973-588-4884
+1-514-316-2339

Get In Touch

    Request a Demo View my Favorites
    Library

    Protein Purification in Downstream Processing

    Describes the use of various chromatographic methods in downstream protein purification including size exclusion, ion exchange, hydrophobic interaction and affinity chromatographies. The basics of a chromatography set-up are covered along with critical factors affecting protein separation such as column packing, resolution, column capacity, pressure and the gel matrix.

    Whether for onboarding or annual refresher training, this Module is seamlessly deployable on any LMS and can be tailored to your company’s exact needs.

      Duration: 30 Mins

    Add to Favorites
    SKU: BPU-1110-SPL05-EN Category: Tag:

    Description

    Learning Objectives:

    • Describe a general outline of a protein purification process for both intracellular and extracellular proteins
    • Describe where chromatography fits into downstream processing
    • Explain the role of different chromatographies in protein purification
    • Describe the difference between Size Exclusion Chromatography, Ion-Exchange Chromatography, Hydrophobic Interaction Chromatography, and Affinity Chromatography
    • Explain what is meant by preparative chromatography
    • Explain terms such as solubility, charge, molecular size, adsorption properties and binding specificity

    Learner Benefits:

    • Develop a deep understanding of the entire protein purification process, focusing on the critical role that chromatography plays in downstream processing for both intracellular and extracellular proteins.
    • Learn the principles and applications of different chromatographic methods, including ion exchange, hydrophobic interaction, size exclusion, and affinity chromatography, and how they contribute to high-resolution separation and product yield.
    • Understand the factors affecting chromatography such as column packing, gel properties, and process parameters, and how these can be used to optimize the yield and purity of biopharmaceutical products.
    • Build a robust technical vocabulary related to chromatography and protein purification, enhancing your ability to communicate effectively in this area.
    		 Module Features:

        Animations
       Voice Over
        Knowledge Checks
        Assessments
       SCORM/AICC compatible
        Full Screen

    Related Modules

    Back To Top