Skip to content

Founded in 1998 in Dublin, Ireland, Skillpad's team of industry-experienced Subject Matter Experts and Consultants believes Pharma, Biopharma, and Life Science Industry manufacturing companies can achieve production excellence with improved performance, productivity & compliance, as well as reduced losses & down-time, if they have KNOWLEDGE CONSISTENCY in all critical areas of manufacturing operations.

With 25 years in business, and offices in Europe, the USA and Canada, Skillpad has helped customers all over the world with Life Science Industry Knowledge challenges. We are fully dedicated to the idea that Knowledge Consistency is more achievable than ever using the latest technology and advanced knowledge transfer and management tools.


Get In Touch

    Protein Purification in Downstream Processing

    Describes the use of various chromatographic methods in downstream protein purification including size exclusion, ion exchange, hydrophobic interaction and affinity chromatographies. The basics of a chromatography set-up are covered along with critical factors affecting protein separation such as column packing, resolution, column capacity, pressure and the gel matrix.

      Duration: 30 Mins

    SKU: BPU-1110-SPL05-EN Category: Tag:


    Learning Objectives:

    • Describe a general outline of a protein purification process for both intracellular and extracellular proteins
    • Describe where chromatography fits into downstream processing
    • Explain the role of different chromatographies in protein purification
    • Describe the difference between Size Exclusion Chromatography, Ion-Exchange Chromatography, Hydrophobic Interaction Chromatography, and Affinity Chromatography
    • Explain what is meant by preparative chromatography
    • Explain terms such as solubility, charge, molecular size, adsorption properties and binding specificity
    Module Features:

       Voice Over
        Knowledge Checks
       SCORM/AICC compatible
        Full Screen

    Back To Top